Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content

Effect of time of harvest on hypericin and essential oil content of hypericum performatum L.

In vitro shoot Proliferation of Hypericum perforatum L. through Indirect and Direct Plant Regeneration

Hypericum perforatum L. (St. Johns’ wort) is the most commercially important species of the genus Hypericum and contains a wide range of components including naphthodianthrones, phloroglucinols, tannins, xanthones, phenolic acids and essential oil. In order to establish an efficient protocol for regeneration, the effects of explant type and plant growth regulators on direct and indirect shoot r...

LC-MS and LC-PDA analysis of Hypericum empetrifolium and Hypericum sinaicum.

Within the framework of our continuous efforts to explore Hypericum species from Jordan, we report the analysis of the major active metabolites, naphthodianthrones and phloroglucinols, in the methanolic extracts of two under-explored Hypericum species; H. empetrifolium Willd. and H. sinaicum Hochst. & Steud. ex Boiss., using LC-(+,-)-ESI-MS (TIC and SIM) and LC-UV/Vis spectroscopy. Based on the...

Hypericin and pseudohypericin in some Hypericum species.

Hypericin and pseudohypericin were found in 27 of the 36 evaluated species from Hypericum L., belonging to 17 sections of the genus. Pseudohypericin is reported by us in 15 taxa for the first time. Most of the species contained both components and the amount of pseudohypericin usually exceeded that of hypericin. In H. hirsutum and H. empetrifolium only hypericin was found, whereas H. formosissi...

Determination of Hypericin Content in Callus and Cell Suspension Cultures of Hypericum triquetrifolium Turra

Hypericum triquetrifolium Turra. is a valuable medicinal plant owing to source of many bioactive compounds. Hypericin is one of the significant components among these compounds. The aim of this study was to develope an efficient method allowing to improve hypericin production from calli and cell suspension cultures of Hypericum triquetrifolium Turra. Callus formation was obtained from axenic le...